Abstract: An improved method for determining available boron in soil of tobacco field was developed, in which soil samples were put into water contained in a common polyethylene bottle, instead of quartz flask and reflux condenser, available boron in soil was extracted in boiling water bath. The boron in the extract was analyzed with inductively coupled plasma emission spectrometry, a substitutional method for curcumine colorimetry. The limit of detection of this method was about 0.0087mg/kg and its relative standard deviation was 6.55%. The contents of boron in two national standard materials, GBW07412 and GBW07414, were determined with the method and the obtained values were close with the standard values. The contents of available boron in 20 soil samples were also determined with the method.