Abstract: The achievements about analysis of organic acids in tobacco at home and abroad over past fifty years were reviewed comprehensively. The properties and contents of various organic acids, such as volatile,semi-volatile and nonvolatile acids, pretreatments including solvent extraction, steam distillation and methyl esterification, and developments and limitations of analytic instruments were compared. It was considered that the exploration of simpler, faster and more accurate analytic methods through improving current methods or developing new ones would be a major subject in analysis of organic acids in tobacco.