Abstract: The activity of sucrose-metabolizing enzymes, such as invertase(Inv),sucrose synthase(SS),and sucrose phosphate syhthase(SPS) in cutters and carbohydrate content in cutters and upper leaves at different growing stages of K326 were experimented in pot by applying different rates of cake fertilizer. The result showed that Inv and SS affected the growth of tobacco leaves at different stages, Inv at metaphase and SS at anaphase. In red sandy-loam soil of Yuxi tobacco growing areas, carbohydrate accumulation of tobacco leaves could be enhanced by 30g cake fertilizer treatment.With the increase of cake fertilizer rates, carbohydrate contents in cutters and upper leaves obviously decreased comparing with those treated with chemical fertilizers.