不同处理对烟草种子萌发及过氧化氢酶活性的影响

Effects of Various Treatments on Germination and Catalase Activity of Tobacco Seeds

摘要: 以烤烟型品种NC89为材料,通过浸种处理研究了不同K⁺浓度、H⁺浓度、渗透势及H₂O₂等对烟草种子萌发过程中呼吸速率和过氧化氢酶活性的影响。0.01~0.1mol/L KNO₃处理对种子萌发过程中活力指数和过氧化氢酶活性有明显的促进作用;0.001~0.01mol/L HCl处理后其活力指数、第4天的过氧化氢酶活性和呼吸速率都比对照高;200~300g/L的PEG-6000溶液浸种24h,对烟草种子活力的提高也有明显效果;0.3% H₂O₂虽能提高烟草种子的呼吸速率,但其发芽势及活力指数均低于对照。

Abstract: Effects of various treatments, such as K⁺, H⁺, osmotic potential and H₂O₂ on respiratory rate and catalase (CAT)activity during germination of flue-cured tobacco cv. NC89 seeds were determined. The results showed that:1)the vital index and CAT activity of seeds treated with 0.01-0.1 mol/L KNO₃ were obviously improved; 2) the vital index, the 4th day's CAT activity, and respiratory rate of seeds treated with 0.001-0.01 mol/L HCl were higher than those of control; 3)the vitality of seeds immersed in 200 -300g/L PEG-6000 for 24 hours was apparently increased; and 4)although the respiratory rate of seeds treated with 0.3% H₂O₂ was promoted, their germinating viability and vital index were lower than those of control.