Abstract: The yield and quality characteristics of seeds of flue-cured tobacco cultivars, such as K 346, K 358 and RG11, in different reserve-seed times were studied.The results showed that there were significant differences in the yield and quality of tobacco seeds, the seed of the flower opening in the second week after central florescence was the best, in the third week was better, the forth week worse and the first week worst.The agronomic characteristics of the three cultivars' seeds showed no significant difference.