烟叶中有机酸的分析

Determination of Organic Acids in Tobacco Using Derivatization Solid-Phase Microextraction and Gas Chromatography-Mass Spectrometry

摘要: 应用硫酸催化对烟叶中有机酸进行甲酯衍生化 ,采用固相微萃取 (SPME)吸附 ,通过气相色谱分离鉴定。分析了 3种烟叶 ,共鉴定了 4 0多种挥发及半挥发性有机酸。为了比较 ,用 2 0g烟叶按常规萃取酯化方法进行GC/MS分析。通过烟叶的分析 ,体现了该方法在烟叶有机酸分析中的快速与简便性。其中 ,对 3-甲基丁酸、苯乙酸、辛酸、十四酸、十六酸进行了定量分析。该方法应用于烟叶中有机酸的定量分析 ,所需时间少 ,能在很短的时间内提供工艺配方及加香加料所需的参考数据。

Abstract: Volatile and semi-volatile organic acids from flue-cured tobacco are converted to their esters by using sulfuric acid & methanol.3-Methy butanoic acid,benzeneacetic acid,octanoic acid,tetradecanioc acid,and hexadecanoic acid are quantitated using derivatization,headspace solid-phase micro-extraction(SPME), and gas chromatography with selected ion monitoring mode mass spectrometry.More than forty organic acids are detected by using this technology.Meanwhile,the volatile and semi-volatile organic acids in the same tobacco samples are analysed with the conventional derivatization and extraction methods and only tweleve organic acids are detected.