芸香苷与铝的显色反应研究及其应用

Study on Color Reaction of Rutin with Al(Ⅲ)and Its Application

摘要: 为了测定烟叶中的铝含量,研究了芸香苷与铝的显色反应。在pH=5.6 HAc-NaAc缓冲介质中和溴化十六烷基三甲基胺(CTMAB)存在下,芸香苷与铝反应生成稳定的络合物。该络合物的λmax=430nm,ε=4.45×10⁴L·mol^-1·cm^-1,铝含量在0~10μg/25ml内符合比尔定律,结合微波消化样品技术,建立了一种烟草样品中铝含量测定的新方法。平行5次测定,该方法的RSD在1.8%~2.4%之间,标准回收率为96%~102%,说明该法可用于烟叶中的铝含量测定。

Abstract: The color reaction of rutin with Al(Ⅲ)was studied.A stable yellow complex was formed while rutin reacted with Al(Ⅲ)in the pH=5.6 HAc-NaAc buffer solution with cetyltrimethylammonium bromide (CTMAB).The molar absorptivity of the complex was 4.45×10⁴ L·mol^-1·cm^-1 at 430nm.The absorptivity of the complex was fitted in Beer's law in the concentration range of 0~10μg Al(Ⅲ)/25ml.A new method for determining the content of Al in tobacco was established by testing absorptivity of the complex.The relative standard deviation and recovery of this method were about 1.8%~2.4% and 96%~102%, respectively by five replicates.