Abstract: The polybasic and higher fatty acids, such as oxalic, malic, citric, palmitic, linoleic and stearic acids in cut tobacco of cigarettes were quantitatively analyzed with methyl esterification, extraction and GC. Tobacco powder containing adipic acid (internal standard)was esterified in H₂SO₄-CH₃OH at room temperature for 22 hrs, followed by extraction with CH₂Cl₂ for 3 times, washed with aqueous saturated NaCl solution, dried over anhydrous Na₂SO₄, and determined by GC. The organic acids in cut tobacco of 11 brands of cigarette were determined and compared. The results showed that 1)the content of malic, oxalic, citric and linoleic acids in cut tobacco of all the brands of cigarette tested was much higher than that of their other organic acids, 2)the concentration of citric and oxalic acids in the blended cigarettes was significantly grearer than that in the flue-cured cigarettes, and 3)the level of palmitic and linoleic acids in the blended cigarettes was lower than that in the flue-cured cigarettes.