Abstract: A method for rapidly determining nine nutrient elements(B,Mg,K,Ca,Fe,Cu,Mn,Zn and Mo) in fresh tobacco leaves by inductively coupled plasma mass spectrometry was developed,and the contents of those elements in leaves were determined at different growing stages.The results showed that:1) The limits of detection ranged from 0.015 to 2.24 mg/kg,and the spiked recoveries were from 94% to 104% with the precision(RSD) of 0.51% to 4.60%.2) The contents of the nine elements in leaves of different growing stages differed obviously,leaves required more nutrient at fast growing stage,and the contents of those nutrient decreased to a certain degree when leaves were mature and harvested.The leaves harvested from different stalk positions in the order of contents of Mg,K,Ca,Fe and Mo were upper leaves>middle leaves>lower leaves,in the order of B and Mn contents and Cu and Zn contents were middle leaves>lower leaves>upper leaves and lower leaves>middle leaves>upper leaves,respectively.