Abstract: In order to investigate the influence of glucose on the formation of hydrogen cyanide(HCN) from asparagine pyrolysis,the pyrolysis products formed from the pyrolysis of asparagine and its mixture with glucose,including HCN,NH₃ and HNCO,were analyzed real-timely by TG-FTIR,and the other volatile products were determined with Py-GC/MS.In addition,by the pyrolysis of ¹⁵N-labeled asparagine sample and the percentage of isotope in the major nitrogen-containing pyrolysis products,their formation pathways were deliberated.The results indicated that:1) When the mixture of asparagine and glucose(1:1 by weight) was pyrolysed,the yield of HCN increased,while the yields of NH₃ and HNCO decreased comparing with the pyrolysis products of asparagine.2) The yields of the major pyrolysis products of asparagine changed in the presence of glucose.The yield of maleimide,the predominant pyrolysis product of asparagine decreased significantly,the yields of succinimide increased,nitriles,acids and amides increased slightly.In addition,a large number of N-heterocyclic compounds emerged.3) With the participation of glucose,the proportion of ¹⁵N in nitrile and amide compounds increased obviously,and that in HCN increased from 27% to 38%.The nitrogen of most N-heterocyclic compounds mainly originated from unlabeled amino group,which indicated that the amino group of asparagine might be more likely to react with the carbonyl group of glucose to form N-heterocyclic compounds,while the amide nitrogen mainly involved in the formation of amides and nitriles.Consequently,the intra-molecular dehydration and cyclization reactions were restrained and the C-C rupture of asparagine intensified,which led to the increase of HCN yield.