Abstract: In order to develop a new natural tobacco flavor,mimose extract was prepared with ultrasonic circulation extraction.The effects of ultrasonic power,extraction time and temperature on the yield of the extract were investigated,and the extracting conditions were optimized by orthogonal experiment.The volatile components in the extract were identified with SPME-GC/MS technology,and the flavoring experiments of the extract on cigarettes were also performed.The results demonstrated that:1) The optimal extracting conditions were:extracting time 30 minutes,ultrasonic power 550 W and extracting temperature 55℃,under which the yield of the extract was 9.83%.2) Sixty-six volatile components were identified in the extract.Among them,the contents of palmitic acid(8.032%),ethyl palmitate(6.807%),hexahydrofarnesyl acetone(6.032%) were relatively higher.3) Mimose concrete could modify and improve cigarette smoke,enhance aroma quality and aftertaste,reduce irritation,and mask offensive odor.