烟草中一种糖苷的分离鉴定及其热解产物

Isolation and Identification of a New Glycoside in Tobacco and Analysis of its Pyrolytic Products

摘要: 通过95%乙醇提取,常压和高压制备色谱分离,从红花大金元烟叶中提取分离得到化合物Ⅰ,利用质谱和核磁共振技术鉴定了化合物Ⅰ的结构,并在氦气中进行了热裂解-GC/MS分析。结果表明:①化合物Ⅰ为(E)-N-(4-羟基-3-甲氧基)苯乙基-3-(3-甲氧基-4-β-D-吡喃葡萄糖)苯基丙烯酰胺;②化合物Ⅰ的热解产物主要是4-乙烯基愈创木酚、愈创木酚、香兰素、对甲基苯酚、2,3-二氢苯并呋喃、4-烯丙基苯酚和3-乙基苯酚。

Abstract: A new glycoside,(E)-N-(4-hydroxy-3-methoxy)-phenethyl-3-(3-methoxy-4-O-β-D-glucopyranosyloxy) phenylacrylamide(Ⅰ), was isolated from leaves of flue-cured tobacco cv."Hongda" through extraction with 95% ethanol,preparation at normal and high pressure and separation with chromatography. The molecular structure of compound(Ⅰ) was identified with HRESI-MS and NMR. Compound(Ⅰ) was pyrolyzed in He atmosphere and its pyrolytic products were analyzed by GC/MS. The results showed that the separated product was compound (Ⅰ),and its major pyrolytic products were 4-vinylguaiacol,guaiacol,vanillin,p-cresol,2, 3-dihydrobenzofuran, 4-allylphenol, and m-ethylphenol.