Abstract: A method for determining the main neutral aroma components in tobacco was developed by combining solvent extraction with heart-cut multidimensional gas chromatography-mass spectrometry (MDGC-MS). Tobacco sample(0.5 g)was extracted by ethyl ether(5 mL), the extract was washed with 5% H₂SO₄(2 mL)subsequently, then analyzed by MDGC-MS after standing overnight. The neutral aromatic components,including solanone, β-damascenone, geranylacetone, β-ionone, megastigmatrienone and dihydroactinidiolide were determined qualitatively and quantitatively by using methyl eugenol as the internal standard. Further,this method was compared with simultaneous distillation extraction(SDE)-gas chromatography-mass spectrometry(GC/MS). The results showed that:The detection limit of the analytes ranged from 0.02 to 0.18μg/mL, the recoveries were between 80.0% to 123.4% with the intra-day relative standard deviation(RSD) ≤7.5%. The developed method is simple, fast, accurate and suitable for the analysis of neutral aroma compounds in batches of tobacco samples.