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凉山和宜宾烤烟烟叶中特征挥发性物质的聚类分析与产地识别

Cluster Analysis of Characteristic Volatile Component in Flue-cured Tobacco Leaves from Liangshan and Yibin and Producing Area Discrimination of the Same

  • 摘要: 为了探寻有助于识别不同产区烤烟烟叶中的特征挥发性香气物质,对采自四川省凉山州(宁南、德昌、普格、会东、会理、盐源、冕宁和越西县)和宜宾地区(兴文、珙县和筠连县)共11县的烤烟烟叶中的挥发性物质进行了气相色谱-质谱(GC/MS)分析和聚类分析。结果表明:①经GC/MS定性和定量分析得到了样品共有的46种挥发性物质,以此作为变量指标进行聚类分析可将样品分为凉山南部、凉山北部和宜宾3个产区类;②从凉山南部和宜宾产区样品的挥发性物质中筛选出7种相对含量较高且较稳定的特征物质(烟碱、法尼基丙酮、棕榈酸、喇叭茶醇、香叶醇、α-西柏三烯二醇和β-西柏三烯二醇),以此作为变量指标对这两个产区的样品再次进行聚类分析,所得结果与用46种挥发性物质聚类分析的结果基本一致。烟叶中相对含量较高且较稳定的特征挥发性物质可用于烟叶的产地识别。

     

    Abstract: In order to recognize the producing areas of flue-cured tobacco leaves, the characteristic volatile aroma components in the leaf samples collected from 11 counties in Liangshan and Yibin, including Ningnan, Dechang, Puge, Huidong, Huili, Yanyuan, Mianning, Yuexi, Xingwen, Gongxian, and Junlian, were analyzed by GC/MS, and then the cluster analysis was performed. The results showed that:1) 46 of the identified volatile components were common to all the samples and were treated as the variables in cluster analysis. The samples were clustered into three groups by producing areas i.e. southern Liangshan, northern Liangshan and Yibin. 2) 7 volatile components (nicotine,farnesyl acetone,palmitic acid,ledol,geraniol,α-cembratriene-diol and β-cembratriene-diol) of higher relative contents and more stable in the samples from southern Liangshan and Yibin were selected as the variables for further cluster analysis, and the results were basically the same as those with the 46 volatile components. It was believed that the characteristic volatile components with higher relative contents and more stable in leaves could be used for the discrimination of producing areas of tobacco.

     

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