Abstract: An effective method for detecting 202 pesticide residues (221 components) in tobacco was developed by PTV-GC-MS/MS (Programmed temperature vaporization sampling technology-gas chromatography-tandem mass spectrometry). The pesticide residues in tobacco sample were extracted rapidly and purified with QuEChERS technique, then separated on TR-pesticideⅡ capillary chromatographic column after PTV sampling. The interference of complex matrix and peak overlapping of various pesticide components were reduced by means of optimizing tandem mass spectrometry parameters. Qualitative and quantitative analyses were conducted by retention time window and SRM (selective reaction monitoring) with peak area respectively. The results showed that:1) The 202 pesticides kept good linear relationships in the range of 0.01-2.50 mg/L with the correlative coefficients between 0.9800 and 0.9999. The limits of detection (LOD) were from 0.0002 to 0.0100 mg/kg, and the recoveries of added standards ranged from 67.4% to 112.0% with the relative standard deviations (RSD) of 0.30%-20.30%. 2) This method was verified with 44 samples of imported tobacco leaves, and results were satisfied. 3) This method covered a wide range of pesticides, featured the advantages of simple and rapid in pre-treatment, solvent saving and capable of analyzing 221 components accurately and sensitively within 46 minutes by a single sample injection.