Abstract: For simultaneous determination of four aromatic amines, including 1-naphthylamine, 2-naphthylamine, 3-aminobiphenyl and 4-aminobiphenyl, in mainstream cigarette smoke, a liquid chromatography tandem mass spectrometric (LC-MS/MS) method was developed. The contents of the four aromatic amines in mainstream smoke of domestic and foreign cigarettes with different tar deliveries were determined with the developed method, and the resultant were compared with that determined with GC-MS method. The results showed that:1) The limits of detection of LC-MS/MS method ranged from 0.016 to 0.038 ng/mL with the recoveries from 70.5% to 116.5%, and the intra-day and inter-day precisions were less than 6% and 9%, respectively. 2) The resultant of LC-MS/MS method well correlated with that of GC-MS method. The developed method was fast, simple, sensitive and suitable for the simultaneous determination of the said aromatic amines in mainstream cigarette smoke.