Abstract: The comparison and cluster analysis of Cr, Ni, Cu, As, Cd, Tl and Pb contents in 100 samples of flue-cured tobacco leaves from 100 areas in 10 provinces of China were conducted for investigating the content and distribution of heavy metals in flue-cured tobacco leaves from major producing areas of China. The results showed that:1) The contents of Pb, Cr, Ni, As in most samples matched the standards set for "environment-friendly"tobacco leaf, while the content of Cd in 52% of the samples fell out of the set standard. 2) The change trends of the said metals were the same for the samples from Hubei, Yunnan, Guizhou, Sichuan and Fujian, and those in the samples from Shaanxi and Liaoning varied in the same way, while those in the samples from Henan, Heilongjiang and Hunan changed differently from each other. 3) Among the said metals, the content of Cu was the highest, those of Cr and As were lower, while that of Tl was the lowest. 4) The Cd content in samples from Hunan, Hubei, Yunnan, Guizhou, Sichuan and Fujian was higher than those from Shaanxi, Henan, Liaoning and Heilongjiang. 5) The 100 samples could be clustered into four categories according to the contents of the said heavy metals. Samples from Guizhou and Sichuan, from most areas of Yunnan, Hubei, Fujian, Shaanxi and Heilongjiang and some areas of Hunan and Henan were clustered into Category Ⅰ, samples from Liaoning and some areas of Heilongjiang, Hubei, Shaanxi and Yunnan into Category Ⅱ, from some areas of Hunan, Henan and Fujian into Category Ⅲ, and only Wuping County in Fujian belonged to Category Ⅳ.