Abstract: The main volatile and semi-volatile components in samples of eight tobacco flavors of two different batches and their dilutions and mixtures were separated and determined by GC/MS after being dissolved by anhydrous ethanol.The GC/MS fingerprint spectra of the flavors were established.The similarities between different samples were calculated with included angle cosine and Euclidean distance ratio methods on the basis of the areas of the peaks,which represented the main volatile and semi-volatile components in the chromatograms of samples.The results showed that:1) This method was simple,fast and suitable for the rapid determination of components in tobacco flavors,the relative standard deviations of all peak areas were less than 6%.2) The samples from different batches showed higher similarity.Either included angle cosine or Euclidean distance ratio was capable of representing the differences of main volatile and semi-volatile components between different samples to a certain extent,however Euclidean distance ratio better represented the concentration differences between samples.