摘要: To elucidate the expression pattern and function of NtCRTISO in Nicotiana tabacum, the transcriptional expression of NtCRTISO in different stages (seedling, growing, flower budding and flowering) and organs (root, stem, leaf and flower) was investigated by real-time PCR. In addition, the expression of NtCRTISO was silenced through VIGS (virus-induced gene silencing) for the investigations described below. The contents of carotenoids (β-carotene, violaxanthin, neoxanthin and lutein), chlorophyll a and chlorophyll b were determined. Furthermore, the levels of chlorophyll fluorescence parameter (Fv/Fm) and non-photochemical quenching (NPQ) were also determined under low temperature and poor light stress conditions. These results showed that NtCRTISO had highly stable expression levels in leaves and the highest level of expression was found in flower budding stage. The expression levels were decreased in descending order in leaf, flower, stem and root. Compared with the control group, the contents of carotenoids, chlorophyll a and chlorophyll b were remarkably increased. Moreover, under low temperature and poor light stress the rate of Fv/Fm and NPQ were decreased. The research results on NtCRTISO would provide useful theoretical basis for tobacco breeding.