摘要: Bacterial wilt caused by Ralstonia solanacearum is one of major diseases for flue-cured tobacco production in Southern China. Ralstonia solanacearum is a complex species that infects more than 200 plant species belonging to more than 50 botanical families, including important crops such as potato, tomato, pepper, tobacco and banana. It is difficult to prevent and control the bacterial wilt of crops because the pathogen Ralstonia solanacearum contains many virulence factors and pathogenesis pathways. The purpose of this study was to investigate the potential of using phages to infect Ralstonia solanacearum, and thus, control the pathogenesis of Ralstonia solancearum. The phage ϕPB2 was further isolated from the rhizosphere of flue-cured tobacco roots from the soils of tobacco-planting field located in Guizhou province, which exhibited a broad range of host for Ralstonia solanacearum strains isolated from flue-cured tobacco in different counties in Guizhou province. Phage-host interaction experiments showed that phage ϕPB2 was infectious and lytic against Ralstonia solanacearum strains Rs1.70 and Rs1.74, which were isolated from peanuts and potatoes respectively. Plaque assay indicated that the titer of phage ϕ PB2 reached 108 pfu(plaque-forming unit)/mL, and phage ϕPB2 was stable in a wide range of temperatures between 4 ℃ and 45 ℃, and could survive up to 50 days in water or tobacco plants after inoculation at environmental temperature. These results suggested that phage ϕPB2 might be used as a potential agent in controlling the bacterial wilt of flue-cured tobacco.