摘要: The matrix effects related to extracting solvents, purification adsorbents, and the target concentration for 22 representative pesticides were investigated using an accelerated solvent extraction and liquid chromatography-tandem mass spectrometry (ASE-LC-MS/MS) method. Two approaches were compared: an extraction-addition method and a gravimetric method. The study results showed that the impact of the extracting solvents on the matrix effect depended on chemical characteristics of co-extractives, i.e., the relative capability of ionization of the co-extractives compared to the targets in ion source. Secondly, the effects of the purification adsorbents on the matrix effect depended on what kinds of and how much co-extractives the adsorbents could remove. The matrix effect could be reduced when the co-extractives removed by the adsorbent were easier to be ionized than the targets. Thirdly, the relationship between the matrix effect and the concentration of target indicated that the matrix effects increased proportionally with the concentration of each target, which reflected the competing equilibrium between the co-extractives and the target compound in ion source. Results presented here provided useful information for reducing the matrix effects in developing new analytical methods for pesticide determination in tobacco.