Development of real-time fluorescence quantification PCR system for detection of Alternaria alternata in tobacco leaves

In order to developed a SYBR Green I real-time fluorescence quantitative PCR system for rapidly detecting Alternaria alternata in tobacco leaves, a pair of specific primers were designed according to the sequence differences of ITS of Alternaria.By constructing a recombinant plasmid containing the targeted fragments, the q-PCR rapid detection system was developed with its standard curve to detect A. alternata-infected tobacco leaves. The sensitivity of detection was above 1×10^-3 ng/μL, which indicated that this system could ensure efficient detection and quantification of A. alternata in tobacco leaves.