Studies on Fermentation Conditions for Nicotine Dehydrogenase Producing Arthrobacter Z3

Arthrobacter Z3,which produces nicotine dehydrogenase,was isolated from the soil in a disposal site of tobacco dust in the cigarette factory.The culture medium of Z3 was optimized with orthogonal experiment,and its fermentation conditions,including initial pH value,inoculum amount,temperature,rotation speed and fermentation time,were tested.According to orthogonal experiment,the optimum medium composition was 2.5% tobacco dust extract,0.1% soybean powder,0.1%(NH₄)₂SO₄,1.33% K₂HPO₄·3H₂O,0.4% KH₂PO₄,0.02% MgSO₄·7H₂O,0.004% MnSO₄·4H₂O,and 0.002% CaCl₂·2H₂O,and the optimum fermentation conditions were:fermenting for 9 h at 30℃,rotation speed of the shaker 220 r/min,initial pH 7.0,and inoculum amount 6%.