Construction of PVY and CMV Binary Virus Resistant RNA Silencing Vector

Using RT-PCR method, the conserved fragment of PVY CP gene, PVY HC-Pro gene, CMV CP gene and CMV 2b gene were cloned, then the fragments of PVY CP and CMV 2b, PVY HC-Pro and CMV 2b, PVY HC-Pro and CMV CP were connected respectively. Three RNA silencing vectors, pCAMPb, pCAMHb and pCAMHP, were constructed using the connected fragments as the target, and the constructed vectors could be used in plant transgenic virus resistance breeding.