Screening,Identification and Degradation Characteristics of High Concentration Nicotine Degradation Bacterium Strain

Utilizing nicotine as the sole source of carbon and nitrogen,152 bacterial strains were isolated from tobacco growing soils in Zibo,Shandong Province and tobacco waste from Wuhan Cigarette Factory,respectively,and a high concentration nicotine degradation bacterium strain ZB-16A was screened out from them.Studies of normal morphology,physiological and biochemical characterizing,16S rDNA gene sequence and phylogenesis tree analyses indicated that strain ZB-16A belongs in Pseudomonas putida.ZB-16A tolerates 6 000 mg/L nicotine concentration and completely degrades nicotine in 48 hours at 30℃ when initial pH of culture medium and rotation speed of shaker are 7.0 and 150 rpm,respectively;while the growth of ZB-16A is inhibited at an initial nicotine concentration of 7000 mg/L.