Determination of Main Active Compounds in Tincture of Radix angelica Pubescens with HPLC-DAD-MS

A method for determining the main active compounds,including columbianetin acetate,osthole,columbianadin and columbianetin,in the tincture of Radix angelica Pubescens with HPLC-DAD-MS was established to control the quality of the tincture.The results indicated that the combination of MS and DAD was suitable for qualification of the four compounds,while DAD was suitable for quantification.The RSDs of these compounds were below 2%,the recovery of osthole was 104.7% with the limit of detection of 1.093 μg/mL.