ISSR Polymorphism Analysis and Molecular Marker of Flavorful Tobacco Leaf in Yunnan's Branded Cigarette

In order to investigate the genetic diversity of flavorful tobacco in branded cigarette produced in Yunnan,7 primers screened from 27 ISSR primers were used for PCR amplification in 17 tobacco samples separately.The results showed that 40 bands were amplified and 28 of them were polymorphic.By cluster analysis based on ISSR markers using UPGMA,the 17 varieties were clustered into 2 major groups.The genetic similarity index was 0.50 to 1.00 among the 17 varieties,each of the varieties could be distinguished from the others by its unique fingerprint using one ISSR primer of strong polymorphism by polyacrylamide gel electrophoresis.It indicated that ISSR analysis was suitable for varietal identification and genetic diversity analysis of tobacco germplasm.