Cloning and Sequence Analysis of T-psy1 Gene from Flue-cured Tobacco

Adopting a homology cloning method,the full length cDNA sequence of tobacco phytoene synthase gene(named T-psy1) was cloned from flue-cured tobacco cv.K326,the T-psy1 gene,GenBank accession number HM345582,included 1521bp,with coding protein containing 441 amino acids,protein accession number ADK25054.The results of BLAST search and phylogenetic analysis showed that T-psy1 presented high genetic homologies with the phytoene synthase of ornamental tobacco,tomato and pepper,which were 95%,91% and 86%,respectively;and the coding protein consistences of T-psy1 gene with phytoene synthase gene of ornamental tobacco,tomato and Elaeagnus umbellate were 97%,90% and 74%,respectively.