ZHOU Xiaolong, WEI Chunyang, HUANG Jia, SHI Yanmei, WANG Hao, LUO Zhaopeng, LI Feng, WANG Ran, YANG Jun, CHEN Jifeng, LIN Fucheng. Regulating Expression of Cu/Zn-SOD Gene in Nicotiana tabacum with miRNA398J. Tobacco Science & Technology, 2014, 47(4): 99-102. DOI: 10.3969/j.issn.1002-0861.2014.04.019
Citation: ZHOU Xiaolong, WEI Chunyang, HUANG Jia, SHI Yanmei, WANG Hao, LUO Zhaopeng, LI Feng, WANG Ran, YANG Jun, CHEN Jifeng, LIN Fucheng. Regulating Expression of Cu/Zn-SOD Gene in Nicotiana tabacum with miRNA398J. Tobacco Science & Technology, 2014, 47(4): 99-102. DOI: 10.3969/j.issn.1002-0861.2014.04.019
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Regulating Expression of Cu/Zn-SOD Gene in Nicotiana tabacum with miRNA398

    Abstract
  • The miRNA398 in model plants could repress the expression of Cu/Zn-SOD gene.In order to reveal the biological function of miRNA398,fluorescent quantitative PCR(qPCR) technology was employed to analyze the expression levels of miRNA398 and Cu/Zn-SOD gene in cv.CB-1 and B37 under drought stress.Meanwhile,the locus where miRNA398 combining with Cu/Zn-SOD gene was also predicted.The results showed that miRNA398 was complementary to a 21 bp sequence in 5'untranslated region of Cu/Zn-SOD gene,thereby to repress the expression of Cu/Zn-SOD gene.Under drought stress,the expression level of miRNA398 was up-regulated in CB-1 and down-regulated in B37,while that of Cu/Zn-SOD gene was on the contrary;which indicated that miRNA398 repressed the expression of Cu/Zn-SOD gene and responsed to drought stress in two different ways in the two varieties of different resistance to drought.
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