Study on the Methods for Testing GMT

DNA of the transgenic tobacco was extracted and its coneentraction and purity in the extract wasestimated by measere of OD at 260~280nm with UV-Spectometer. PCR ampilfication of chloroplast nonconding DNA region Sequence also be used to check the quality of DNA in the extract, then PCR amplification for 35S promoter and NOS terminator was carried out simultaneously. Nested PCR of 35S and NOS was conducted for nospecific PCR band in the screening. DNA was re-extracted and PCR screening was repeated for the sample withoutspecific PCR in the screening. Restriction enzyme analysis of 35S and NOS PCR products, nested PCR, probe hybrid and amplification for GMT target gene were taken to confirm the screening results and determine the kind of target transgene and measure the content of GMT with cmpetitive PCR system.